Establishment and characterization of a new human Ph+ erythroleukemic cell line, VES
Continuous human malignant hematopoietic cell lines are invaluable tool for hematological research. Here we report on a new erythroleukemic cell line termed VES that was established from the bone marrow mononuclear cells (BMNC) of a 22-year-old woman with Ph+ chronic myeloid leukemia (CML). During s...
| Permalink: | http://skupni.nsk.hr/Record/ffzg.KOHA-OAI-FFZG:314772/Details |
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| Matična publikacija: |
Book of Abstracts 2005 |
| Glavni autori: | Golemović, Mirna (-), Rnjak, Lana (Author), Dubravcic, Klara Davidovic-Mrsic, Sanja, Franic, Ivana Ries, Suncica, Gjadrov, Koraljka Aralica, Merica, Aurer, Igor Mitrovic, Zdravko, Serventi Seiwerth, Ranka Labar, Boris, Batinic, Drago |
| Vrsta građe: | Članak |
| Jezik: | eng |
| LEADER | 03908naa a2200301uu 4500 | ||
|---|---|---|---|
| 008 | 131111s2005 xx 1 eng|d | ||
| 035 | |a (CROSBI)214494 | ||
| 040 | |a HR-ZaFF |b hrv |c HR-ZaFF |e ppiak | ||
| 100 | 1 | |a Golemović, Mirna | |
| 245 | 1 | 0 | |a Establishment and characterization of a new human Ph+ erythroleukemic cell line, VES / |c Golemović, Mirna ; Rnjak, Lana ; Dubravcic, Klara ; Davidovic-Mrsic, Sanja ; Franic, Ivana ; Ries, Suncica ; Gjadrov, Koraljka ; Aralica, Merica ; Aurer, Igor ; Mitrovic, Zdravko ; Serventi Seiwerth, Ranka ; Labar, Boris ; Batinic, Drago.. |
| 246 | 3 | |i Naslov na engleskom: |a Establishment and characterization of a new human Ph+ erythroleukemic cell line, VES | |
| 300 | |a 39 |f str. | ||
| 520 | |a Continuous human malignant hematopoietic cell lines are invaluable tool for hematological research. Here we report on a new erythroleukemic cell line termed VES that was established from the bone marrow mononuclear cells (BMNC) of a 22-year-old woman with Ph+ chronic myeloid leukemia (CML). During second post-transplant recovery her bone marrow was tested Ph-negative by FISH and PCR and BMNC were introduced into culture. On the day 27 blast-like cells appeared in the supernatant of cell culture. They expressed highly amplified bcr/abl locus as shown by FISH, whereas the patient’ s bone marrow was still Ph-negative at the time. Very soon, the cultured cells started to express stable features of erythroleukemia cell line. Optimal growth was obtained at concentration of 0.3x106 cells/ml in IMDM containing 10% FBS. After 3 days of culture, cell concentration varied between 1.3 and 1.8x106cells/ml. VES cells were tested negative for the presence of EBV. For analysis of clonogenicity, 103cells/ml were grown in a serum-free methylcellulose medium without cytokines. Following 7-day culture, 103 VES cells produced 251± ; ; ; 42 and after 14 days 431± ; ; ; 30 GF-independent colonies. On day 14 it was possible to observe reddish color of the colonies indicating the presence of hemoglobin. Cytological examination revealed homogenous population of leukemia blasts (>80%) the majority of them (>90%) being glycophorin A positive and MPO negative. Immunophenotyping indicated proerythroblastic lineage-associated profile: GlyA+, CD11b+, CD15+, CD29+, CD33+ and CD117+. Cytogenetics revealed complex karyotype with multiple numerical/structural abnormalities (MAKA), while metaphase FISH revealed t(9 ; 22), 5q31, 7q31, +8, +6. Since FISH analysis detected highly amplified bcr/abl locus, we tested VES cells’ sensitivity to imatinib (Gleevec, Novartis). Treatment with imatinib for 3 days (MTS assay) inhibited proliferation of VES cells with IC50 value of 0.2 M. Following 14 days of culture in methylcellulose medium with addition of 0.2 M imatinib, the clonogenic potential of VES cells was reduced by 55%. Furthermore, imatinib induced apoptosis in VES cells in time- and dose-dependant manner, assessed by AnnexinV/PI staining. We believe that VES cells represent a suitable model for studying Ph+ malignant hematopoiesis in vitro. | ||
| 536 | |a Projekt MZOS |f 0214201 | ||
| 546 | |a ENG | ||
| 690 | |a 3.01 | ||
| 693 | |a Ph chromosome, Erythroid, Leukemia cell line |l hrv |2 crosbi | ||
| 693 | |a Ph chromosome, Erythroid, Leukemia cell line |l eng |2 crosbi | ||
| 700 | 1 | |a Rnjak, Lana |4 aut | |
| 700 | 1 | |a Dubravcic, Klara |4 aut |a Davidovic-Mrsic, Sanja | |
| 700 | 1 | |a Franic, Ivana |4 aut |a Ries, Suncica | |
| 700 | 1 | |a Gjadrov, Koraljka |4 aut |a Aralica, Merica | |
| 700 | 1 | |a Aurer, Igor |4 aut |a Mitrovic, Zdravko | |
| 700 | 1 | |a Serventi Seiwerth, Ranka |4 aut |a Labar, Boris | |
| 700 | 1 | |a Batinic, Drago. |4 aut | |
| 773 | 0 | |a Annual meeting of Croatian Immunological society (29.9.-2.10.2005. ; Božava, Dugi otok, Hrvatska) |t Book of Abstracts |d 2005 |g str. 39 | |
| 942 | |c RZB |u 1 |v DomRecenzija |z Znanstveni - Poster - Sazetak | ||
| 999 | |c 314772 |d 314770 | ||