Sequential optimization approach for enhanced production of poly(γ-glutamic acid) from newly isolated Bacillus subtilis
Summary: A bacterial strain of marine origin showing production of poly(γ-glutamic acid) (PGA) has been identified by taxonomical and 16S rRNA studies as Bacillus subtilis. A sequential optimization approach was applied for improving the PGA production. The effect of carbon sources, nitrogen sources...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01000736143/Details |
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| Matična publikacija: |
Food technology and biotechnology 47 (2009), 3 ; str. 313-322 |
| Glavni autor: | Bajaj, Ishwar B. (-) |
| Ostali autori: | Singhal, Rekha S. (-) |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
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| 001 | NSK01000736143 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20121012095346.0 | ||
| 007 | ta | ||
| 008 | 100510s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738767 | ||
| 035 | |a (HR-ZaNSK)000736143 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 57 |2 MRF 1998. | ||
| 100 | 1 | |a Bajaj, Ishwar B. | |
| 245 | 1 | 0 | |a Sequential optimization approach for enhanced production of poly(γ-glutamic acid) from newly isolated Bacillus subtilis / |c Ishwar B. Bajaj and Rekha S. Singhal. |
| 300 | |b Ilustr. | ||
| 500 | |a Congress of Croatian society of biochemistry and molecular biology, Osijek, Croatia, 17-20 September 2008 | ||
| 504 | |a Bibliografija: 33 jed | ||
| 520 | 8 | |a Summary: A bacterial strain of marine origin showing production of poly(γ-glutamic acid) (PGA) has been identified by taxonomical and 16S rRNA studies as Bacillus subtilis. A sequential optimization approach was applied for improving the PGA production. The effect of carbon sources, nitrogen sources and pH on the production of PGA was investigated by one factor-at-a-time method. Plackett-Burman design was then adopted to select the most important nutrients influencing the yield of PGA. After identifying the most significant nutrients, response surface methodology (RSM) was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally. PGA production was further improved by supporting the medium with α-ketoglutaric acid. The PGA production increased from 7.64 to 25.38 g/L by using the sequential optimization methods | |
| 653 | 0 | |a Bacillus subtilis |a Poli(γ-glutaminska kiselina) |a Metoda odzivne plohe | |
| 700 | 1 | |a Singhal, Rekha S. | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 3 ; str. 313-322 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100510 |c vol2o121012 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |