Biocatalytic synthesis of polymeric esters used as emulsifiers

Polyglycerol polyricinoleate (PGPR) is a polymeric ester widely used as emulsifier in the food industry. In this work, PGPR biocatalytic synthesis was carried out in a onestep solvent-free enzymatic process using lipase CALB immobilized in Lewatit® Monoplus MP 64 by adsorption. The optimal immobiliz...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01001068905/Details
Matična publikacija: Chemical & biochemical engineering quarterly (Online)
33 (2019), 1 ; str. 79-86
Glavni autori: Ortega-Requena, Salvadora (Author), Serrano-Arnaldos, M., Montiel Morte, M. Claudia, Máximo Martín, María Fuensanta, Bastida, Josefa, Murcia Almagro, M. D.
Vrsta građe: e-članak
Jezik: eng
Predmet:
Online pristup: https://doi.org/10.15255/CABEQ.2018.1303
Chemical & biochemical engineering quarterly (Online)
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245 1 0 |a Biocatalytic synthesis of polymeric esters used as emulsifiers  |h [Elektronička građa] /  |c S. Ortega-Requena, M. Serrano-Arnaldos, M. C. Montiel, F. Máximo, J. Bastida, M. D. Murcia. 
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504 |a Summary. 
520 |a Polyglycerol polyricinoleate (PGPR) is a polymeric ester widely used as emulsifier in the food industry. In this work, PGPR biocatalytic synthesis was carried out in a onestep solvent-free enzymatic process using lipase CALB immobilized in Lewatit® Monoplus MP 64 by adsorption. The optimal immobilization conditions were determined: initial enzyme concentration of 13 mg of Lowry protein per mL phosphate buffer pH 7, and ricinoleic acid as a support activator. An immobilized derivative with 35.93 ± 4.90 mg of Lowry protein per g of dry support was obtained. It was used as a catalyst for PGPR production in open air and vacuum batch reactors, and the results obtained showed that only when the reaction equilibrium was shifted towards ester production by means of water removal, the PGPR produced fulfilled the European legislation (acid value ≤ 6 mg of KOH per g of product). 
653 0 |a Biokatalitička sinteza  |a Polimeri  |a Esteri  |a Emulgatori  |a Lipaza  |a Imobilizirana lipaza  |a Enzimska aktivnost  |a Troškovi proizvodnje  |a Industrijska primjena 
700 1 |a Serrano-Arnaldos, M.  |4 aut  |9 HR-ZaNSK 
700 1 |a Montiel Morte, M. Claudia  |4 aut 
700 1 |a Máximo Martín, María Fuensanta  |4 aut 
700 1 |a Bastida, Josefa  |4 aut 
700 1 |a Murcia Almagro, M. D.  |4 aut  |9 HR-ZaNSK 
773 0 |t Chemical & biochemical engineering quarterly (Online)  |x 1846-5153  |g 33 (2019), 1 ; str. 79-86  |w nsk.(HR-ZaNSK)000622963 
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