Faecal calprotectin determination
Introduction: We assessed the differences in faecal calprotectin (FC) concentrations measured by two assays depending on the stool consistencyand extraction method.Materials and methods: Stool samples were extracted using the EliA Stool Extraction Kit, Calex® Cap extraction device and respective wei...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001068977/Details |
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| Matična publikacija: |
Biochemia medica (Online) 29 (2019), 1 ; str. 112-122 |
| Glavni autori: | Juričić, Gordana (Author), Brenčić, Tina, Tešija Kuna, Andrea, Njegovan, Milena, biokemičarka, Honović, Lorena |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.11613/BM.2019.010707 Biochemia medica (Online) Hrčak |
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| 008 | 200709s2018 ci a |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.11613/BM.2019.010707 | |
| 035 | |a (HR-ZaNSK)001068977 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 577 |2 2011 | |
| 080 | 1 | |a 61 |2 2011 | |
| 100 | 1 | |a Juričić, Gordana |4 aut | |
| 245 | 1 | 0 | |a Faecal calprotectin determination |h [Elektronička građa] : |b impact of preanalytical sample treatment and stool consistency on within- and between-method variability / |c Gordana Juricic, Tina Brencic, Andrea Tesija Kuna, Milena Njegovan, Lorena Honovic. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 26 jed. | ||
| 504 | |a Abstract. | ||
| 520 | |a Introduction: We assessed the differences in faecal calprotectin (FC) concentrations measured by two assays depending on the stool consistencyand extraction method.Materials and methods: Stool samples were extracted using the EliA Stool Extraction Kit, Calex® Cap extraction device and respective weighingmethods, while FC concentrations were measured using the EliATM Calprotectin and Bühlmann fCAL® Turbo method and checked for within- andbetween-method variability with regard to extraction method and stool consistency category. Extraction yield was evaluated for impact of differentsample incubation time (10 min and 1 h) in extraction buffer for both methods and for impact of different initial sample dilutions (1:50, 1:100, 1:500)for fCAL® Turbo method.Results: Results determined from Calex® Cap extracts were higher compared to weighing method extracts (mean bias 33.3%; P <0.001), whileno significant difference was found between results obtained with EliA Stool Extraction Kit and weighing method (mean bias 0.1%; P = 0.484), inboth cases irrespective of stool consistency. Bühlmann fCAL® Turbo results were higher than EliATM Calprotectin results (mean bias 32.3%, P = 0.025weighing method; and mean bias 53.9%, P <0.001 extraction devices), the difference is dependent on stool consistency and FC concentration. Significantlyhigher FC extraction yield was obtained with longer sample incubation time for both methods (P = 0.019 EliATM Calprotectin; P <0.001fCAL® Turbo) and with increasing initial sample dilution for fCAL® Turbo method (P <0.001).Conclusion: Preanalytical stool sample handling proved to be a crucial factor contributing to within- and between-FC assay variability. Standardizationis urgently needed in order to assure comparable and reliable FC results. | ||
| 653 | 0 | |a Kalprotektin |a Upalne bolesti crijeva |a Imunološki test |a Preanalitička faza |a Laboratorijska dijagnostika | |
| 700 | 1 | |a Brenčić, Tina |4 aut | |
| 700 | 1 | |a Tešija Kuna, Andrea |4 aut | |
| 700 | 1 | |a Njegovan, Milena, |c biokemičarka |4 aut | |
| 700 | 1 | |a Honović, Lorena |4 aut | |
| 773 | 0 | |t Biochemia medica (Online) |x 1846-7482 |g 29 (2019), 1 ; str. 112-122 |w nsk.(HR-ZaNSK)000655372 | |
| 981 | |b Be2018 |b B03/18 | ||
| 998 | |b tino2009 | ||
| 856 | 4 | 0 | |u https://doi.org/10.11613/BM.2019.010707 |
| 856 | 4 | 0 | |u https://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.2019.010707 |y Biochemia medica (Online) |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/217219 |y Hrčak |