Characterization of ceramides with phytosphingosine backbone by hydrogen-deuterium exchange mass spectrometry
Ceramides are a lipid subclass of the sphingolipids that show large structural diversity. Structural characterization of the ceramides (CERs) can lead to better understanding of their role and function in the biological system. Here we investigated representatives of NP (CER III, CER IIIB) and AP ce...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001074278/Details |
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| Matična publikacija: |
Croatica chemica acta (Online) 92 (2019), 3 ; str. 411-417 |
| Glavni autori: | Đapić, Irena (Author), Jakaša, Ivone, Kobetić, Renata, inženjerka kemije, Brkljačić, Lidija, inženjerka kemije |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.5562/cca3506 Hrčak |
| LEADER | 02397naa a22003734i 4500 | ||
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| 001 | NSK01001074278 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20220216152415.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 200916s2019 ci d |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.5562/cca3506 | |
| 035 | |a (HR-ZaNSK)001074278 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 577 |2 2011 | |
| 100 | 1 | |a Đapić, Irena |4 aut | |
| 245 | 1 | 0 | |a Characterization of ceramides with phytosphingosine backbone by hydrogen-deuterium exchange mass spectrometry |h [Elektronička građa] / |c Irena Dapic, Ivone Jakasa, Renata Kobetic, Lidija Brkljacic. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 33 jed. | ||
| 504 | |a Summary. | ||
| 520 | |a Ceramides are a lipid subclass of the sphingolipids that show large structural diversity. Structural characterization of the ceramides (CERs) can lead to better understanding of their role and function in the biological system. Here we investigated representatives of NP (CER III, CER IIIB) and AP ceramide classes (CER VI) that contain phytosphingosine (P) backbone. Ceramides were characterized in positive ionization mode by hydrogen-deuterium exchange mass spectrometry (HDX-MS). Fragmentation in positive ionization mode of the CER III and CER VI resulted in abundant ions assigned to phytosphingosine moiety at m/z 282, 300 and 318. HDX-MS of fragments showed increase in m/z of corresponding ions confirming the exchange of deuterium. In negative ionisation spectra multiple fragment ions were assigned to fatty acyl (RCOO–) moiety. Presence of RCOO– allowed unambiguous identification of CER III and CER IIIB which were distinguished by the presence of double bond on fatty acyl chain. | ||
| 653 | 0 | |a Ceramidi |a Lipidi |a Sfingolipidi |a Sfingozin |a Vodikovi izotopi |a Teški vodik |a Deuterij |a Masena spektrometrija |a Tandemska spektrometrija masa | |
| 700 | 1 | |a Jakaša, Ivone |4 aut | |
| 700 | 1 | |a Kobetić, Renata, |c inženjerka kemije |4 aut | |
| 700 | 1 | |a Brkljačić, Lidija, |c inženjerka kemije |4 aut | |
| 773 | 0 | |t Croatica chemica acta (Online) |x 1334-417X |g 92 (2019), 3 ; str. 411-417 |w nsk.(HR-ZaNSK)000255036 | |
| 981 | |b Be2019 |b B05/19 | ||
| 998 | |b dalo2010 | ||
| 856 | 4 | 0 | |u https://doi.org/10.5562/cca3506 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/226698 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |