Phenotype analysis of CD3+CD16+ lymphocytes in the peripheral blood of pigs
The phenotype of porcine peripheral blood T cells and natural killer (NK) cells has been well-studied over the past three decades, though porcine peripheral blood lymphocytes with mixed T/NK-cell phenotype within perforin- and NKp46- positive CD3+ populations have also been identified. Despite the m...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001084169/Details |
|---|---|
| Matična publikacija: |
Veterinarska stanica (Online) 51 (2020), 2 ; str. 117-128 |
| Glavni autori: | Andrišić, Miroslav (Author), Žarković, Irena, Šandor, Ksenija, Vujnović, Anja, Perak, Eleonora, Valpotić, Hrvoje, Bendelja, Krešo, Cvetić, Željko, Božić, Frane, Terzić, Svjetlana |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.46419/vs.51.2.2 Hrčak |
| LEADER | 05599naa a22004694i 4500 | ||
|---|---|---|---|
| 001 | NSK01001084169 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20201221120714.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 201210s2020 ci d |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.46419/vs.51.2.2 | |
| 035 | |a (HR-ZaNSK)001084169 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 636.09 |2 2011 | |
| 080 | 1 | |a 54 |2 2011 | |
| 100 | 1 | |a Andrišić, Miroslav |4 aut | |
| 245 | 1 | 0 | |a Phenotype analysis of CD3+CD16+ lymphocytes in the peripheral blood of pigs |h [Elektronička građa] / |c Miroslav Andrišić, Irena Žarković, Ksenija Šandor, Anja Vujnović, Eleonora Perak Junaković, Hrvoje Valpotić, Krešo Bendelja, Željko Cvetić, Frane Božić, Svjetlana Terzić. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 31 jed. | ||
| 504 | |a Summary ; Sažetak. | ||
| 520 | |a The phenotype of porcine peripheral blood T cells and natural killer (NK) cells has been well-studied over the past three decades, though porcine peripheral blood lymphocytes with mixed T/NK-cell phenotype within perforin- and NKp46- positive CD3+ populations have also been identified. Despite the mixed phenotype, both populations showed in vitro NK cell-like major histocompatibility complex-unrestricted cytolysis. In this study, the peripheral blood lymphocytes of 15 crossbreed, 12-week-old pigs of both sexes, were analysed by flow cytometry for the expression of leukocyte surface antigens (cluster of differentiation, CD) that can be found on porcine T cells (CD3, TCR-γδ and CD4), NK cells (CD16) or on both cell populations (CD8α and SLA-DR). We found the presence of a minor population of CD3+CD16+ cells within peripheral blood lymphocytes (2.84%). Peripheral blood CD3+CD16+ lymphocytes consisted of all four subpopulations with respect to the expression of surface antigens CD4 and CD8α; most were CD4-CD8α+ (60.64%) and CD4-CD8α-(36.77%). While the proportion of SLA- DR+ cells within both subpopulations was similar (8.01% of CD3+CD16+CD4-CD8α+ lymphocytes and 7.41% of CD3+CD16+CD4- CD8α- lymphocytes), the proportion of TCR-γδ+ cells was noticeably higher within CD3+CD16+CD4-CD8α+ (43.48%) than CD3+CD16+CD4-CD8α- (16.55%) lymphocytes. When the expression of individual surface antigens was analysed on peripheral blood CD3+CD16+ lymphocytes, most were CD8α+ (62.44%), though some were also TCR- γδ+ (32.56%), SLA-DR+ (7.55%) or CD4+ (2.59%). Expression of CD8α on CD3+CD16+ lymphocytes was not related to co-expression of other surface antigens, though most CD3+CD16+TCR-γδ+ lymphocytes (81.04%) and most CD3+CD16+CD4+ lymphocytes (69.50%) expressed CD8α. Expression of SLA-DR was not related to the co-expression of TCR-γδ or CD8α, or to the co-expression of both antigens (TCR-γδ and CD8α) on CD3+CD16+CD4- lymphocytes. | ||
| 520 | |a Fenotip T limfocita i NK stanica (engl. Natural Killer Cells) periferne krvi svinja dobro je istražen tijekom zadnja tri desetljeća. Međutim, unutar subpopulacija limfocita periferne krvi svinja koje iskazuju površinski antigen CD3 i perforin ili receptor NKp46 ustvrđeni su i limfociti s mješovitim fenotipom T limfocita i NK stanica. Unatoč mješovitom fenotipu, obje subpopulacije limfocita su in vitro pokazale citolitički učinak koji nije bio usmjeren na molekule glavnog sustava tkivne podudarnosti, odnosno citolitički učinak sličan NK stanicama. U našem istraživanju protočnom citometrijom analizirani su limfociti periferne krvi od petnaest svinja oba spola, križane pasmine i u dobi dvanaest tjedana, s obzirom na iskazivanje površinskih antigena koji mogu biti prisutni na T limfocitima (CD3, TCR-γδ i CD4), na NK stanicama (CD16) ili na obje navedene populacije stanica svinja (CD8α i SLA-DR). Naše je istraživanje pokazalo da limfociti periferne krvi sadržavaju manji udio subpopulacije koja iskazuje površinske antigene CD3 i CD16 (2,84%). Limfociti periferne krvi s fenotipom CD3+CD16+ sastojali su se od sve četiri subpopulacije s obzirom na iskaziva- nje površinskih antigena CD4 and CD8α, od kojih je većina bila CD4-CD8α+ (60,64%) i CD4- CD8α- (36,77%). Iako je udio stanica koje iskazuju SLA-DR u obje navedene subpopulacije bio sličan (8,01 % od CD3+CD16+CD4-CD8α+ limfocita i 7,41 % od CD3+CD16+CD4-CD8α- limfocita), udio stanica koje iskazuju TCR-γδ je bio primjetno veći u CD3+CD16+CD4-CD8α+ limfocitima (43,48 %), nego u CD3+CD16+CD4- CD8α- limfocitima (16,55 %). Analiza iskazivanja pojedinih površinskih antigena na CD3+CD16+ limfocitima periferne krvi poka- zala je da ih je većina bila CD8α+ (62,44 %), ali također i TCR-γδ+ (32,56 %), SLA-DR+ (7,55 %) i CD4+ (2,59 %). Iskazivanje površinskog antigena CD8α na CD3+CD16+ lim- focitima nije bilo povezano s iskazivanjem drugih površinskih antigena. | ||
| 653 | 0 | |a Svinje |a T-limfociti |a Analiza fenotipa |a NK stanice | |
| 700 | 1 | |a Žarković, Irena |4 aut | |
| 700 | 1 | |a Šandor, Ksenija |4 aut | |
| 700 | 1 | |a Vujnović, Anja |4 aut | |
| 700 | 1 | |a Perak, Eleonora |4 aut | |
| 700 | 1 | |a Valpotić, Hrvoje |4 aut | |
| 700 | 1 | |a Bendelja, Krešo |4 aut | |
| 700 | 1 | |a Cvetić, Željko |4 aut | |
| 700 | 1 | |a Božić, Frane |4 aut | |
| 700 | 1 | |a Terzić, Svjetlana |4 aut | |
| 773 | 0 | |t Veterinarska stanica (Online) |x 1849-1170 |g 51 (2020), 2 ; str. 117-128 |w nsk.(HR-ZaNSK)000855872 | |
| 981 | |b Be2020 |b B01/20 | ||
| 998 | |b dalo2012 | ||
| 856 | 4 | 0 | |u https://doi.org/10.46419/vs.51.2.2 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/236147 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |