Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea

Seasonal sampling of the seawater column and sediment in Adriatic coastal areas affected by various anthropogenic activities, primarily aquaculture, was conducted during 2017. In total, 32 samples from two sites (central and southern Adriatic) were analysed by 16S rRNA amplicon sequencing. This appr...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01001084197/Details
Matična publikacija: Acta botanica Croatica (Online)
79 (2020), 2 ; str. 157-169
Glavni autori: Kolda, Anamarija (Author), Ljubešić, Zrinka, Gavrilović, Ana, veterinarka, Jug-Dujaković, Jurica, Pikelj, Kristina, Kapetanović, Damir
Vrsta građe: e-članak
Jezik: eng
Predmet:
Online pristup: https://doi.org/10.37427/botcro-2020-021
Hrčak
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024 7 |2 doi  |a 10.37427/botcro-2020-021 
035 |a (HR-ZaNSK)001084197 
040 |a HR-ZaNSK  |b hrv  |c HR-ZaNSK  |e ppiak 
041 0 |a eng  |b eng 
042 |a croatica 
044 |a ci  |c hr 
080 1 |a 55  |2 2011 
080 1 |a 502/504  |2 2011 
100 1 |a Kolda, Anamarija  |4 aut 
245 1 0 |a Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea  |h [Elektronička građa] /  |c Anamarija Kolda, Zrinka Ljubešić, Ana Gavrilović, Jurica Jug-Dujaković, Kristina Pikelj, Damir Kapetanović. 
300 |b Ilustr., graf. prikazi. 
504 |a Bibliografija: str. 166-169. 
504 |a Summary. 
520 |a Seasonal sampling of the seawater column and sediment in Adriatic coastal areas affected by various anthropogenic activities, primarily aquaculture, was conducted during 2017. In total, 32 samples from two sites (central and southern Adriatic) were analysed by 16S rRNA amplicon sequencing. This approach was selected to test the possibilities of using metabarcoding in studying marine cyanobacteria, exploring their ecology and potential as an indicator group in anthropologically stressed coastal environments. Additionally, physicochemical water column parameters, sediment granulometry and composition were assessed. Water column revealed a seasonal variation of amplicon sequencing variants (ASVs) closely related to Cyanobium PCC-6307, Prochlorococcus MIT9313 and Synechococcus CC9902, as well as seasonal grouping of physico-chemical parameters in PCA analysis. Sediment analysis uncovered greater community richness of 13 cyanobacterial genera and two uncultured groups. The most abundant in sandy gravels and gravelly sand type of sediments were ASVs closely related to Pleurocapsa PCC-7319 and Xenococcus PCC-7305. Furthermore, identified cyanobacterial ASVs predominantly displayed similarity to isolates from tropical areas (e.g. Neolyngbya, Chroococcidiopsis, Trichodesmium, etc.), which could indicate the tropicalization process already ongoing in the fish fauna of the Adriatic Sea. 
653 0 |a Morska voda  |a Cijanobakterije  |a Ekologija  |a Sedimenti 
653 5 |a Jadransko more 
700 1 |a Ljubešić, Zrinka  |4 aut 
700 1 |a Gavrilović, Ana,  |c veterinarka  |4 aut 
700 1 |a Jug-Dujaković, Jurica  |4 aut 
700 1 |a Pikelj, Kristina  |4 aut 
700 1 |a Kapetanović, Damir  |4 aut 
773 0 |t Acta botanica Croatica (Online)  |x 1847-8476  |g 79 (2020), 2 ; str. 157-169  |w nsk.(HR-ZaNSK)000750349 
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856 4 0 |u https://doi.org/10.37427/botcro-2020-021 
856 4 0 |u https://hrcak.srce.hr/239827  |y Hrčak 
856 4 1 |y Digitalna.nsk.hr