In vitro lead tolerance testing in white poplar genotypes on acidic medium

This study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characte...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01001101645/Details
Matična publikacija: South-east European forestry (Online)
11 (2020), 2 ; str. 153-160
Glavni autori: Kovačević, Branislav, inženjer šumarstva (Author), Tišma, Gordana, Nikolić, Nataša, biolog, Milović, Marina, Vuksanović, Vanja, Orlović, Saša
Vrsta građe: e-članak
Jezik: eng
Predmet:
Online pristup: https://doi.org/10.15177/seefor.20-18
Hrčak
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024 7 |2 doi  |a 10.15177/seefor.20-18 
035 |a (HR-ZaNSK)001101645 
040 |a HR-ZaNSK  |b hrv  |c HR-ZaNSK  |e ppiak 
041 0 |a eng  |b eng 
042 |a croatica 
044 |a ci  |c hr 
080 1 |a 630  |2 2011 
100 1 |a Kovačević, Branislav,  |c inženjer šumarstva  |4 aut 
245 1 0 |a In vitro lead tolerance testing in white poplar genotypes on acidic medium  |h [Elektronička građa] /  |c Branislav Kovačević, Gordana Tišma, Nataša Nikolić, Marina Milović, Vanja Vuksanović, Saša Orlović. 
300 |b Ilustr., graf. prikazi. 
504 |a Bibliografija: str. 159-160. 
504 |a Summary. 
520 |a This study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characters were measured: number of roots, the length of the longest root, the shoot height, dry root mass and dry shoot mass, and parameters related to photosynthetic pigments: content of chlorophyll a, b, a+b, and of carotenoids in fresh mass, as well as chlorophyll a/b ratio. For further statistical analysis, tolerance indices by Turner and Marshal (TI) were calculated for each measured character. The strongest inhibitory effect was achieved on the medium with 10-4 M Pb(NO3)2, but the best differentiation between genotypes was achieved on the medium with 10-5 M Pb(NO3)2. The highest tolerance indices for the length of the longest root and shoot height had genotypes L-12 and LBM, and for root and shoot dry mass genotypes LCM and L-12. There were no significant differences between genotypes in tolerance indices by any of the examined photosynthetic parameters. The obtained results suggest that both low pH and the presence of citric acid as chelating agent improved evaluation of lead tolerance in comparison with the results obtained on media with standard pH and without citric acid in similar studies. In vitro tests on acidic medium with citric acid can efficiently differentiate examined genotypes for lead tolerance, which could be important for their use in phytoextraction projects, especially on acidic soils. 
653 0 |a Bijela topola  |a Populus alba  |a Teški metali  |a In vitro istraživanje  |a Ukorjenjivanje 
700 1 |a Tišma, Gordana  |4 aut  |9 HR-ZaNSK 
700 1 |a Nikolić, Nataša,  |c biolog  |4 aut 
700 1 |a Milović, Marina  |4 aut  |9 HR-ZaNSK 
700 1 |a Vuksanović, Vanja  |4 aut  |9 HR-ZaNSK 
700 1 |a Orlović, Saša  |4 aut 
773 0 |t South-east European forestry (Online)  |x 1849-0891  |g 11 (2020), 2 ; str. 153-160  |w nsk.(HR-ZaNSK)000854680 
981 |b Be2020  |b B03/20 
998 |b dalo2106 
856 4 0 |u https://doi.org/10.15177/seefor.20-18 
856 4 0 |u https://hrcak.srce.hr/248149  |y Hrčak 
856 4 1 |y Digitalna.nsk.hr