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Simple thrombin-based method for eliminating fibrinogen interference in serum protein electrophoresis of haemodialysed patients

Introduction: Serum samples of haemodialysed patients collected through vascular access devices, e.g. central venous catheter (CVC) can contain residual heparin, which can cause incomplete clotting and consequently fibrinogen interference in serum protein electrophoresis (SPE). We hypothesized that...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01001102478/Details
Matična publikacija: Biochemia medica (Online)
30 (2020), 2 ; str. 265-271
Glavni autori: Rade, Anamarija (Author), Đuras, Anamarija, Kocijan, Irena, biokemičarka, Banković-Radovanović, Patricija, Turčić, Ana
Vrsta građe: e-članak
Jezik: eng
Predmet:
Fibrinogen > Elektroforeza > Trombin > Hemodijaliza
Online pristup: https://doi.org/10.11613/BM.2020.020705
Biochemia medica (Online)
Hrčak
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024 7 |2 doi  |a 10.11613/BM.2020.020705 
035 |a (HR-ZaNSK)001102478 
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042 |a croatica 
044 |a ci  |c hr 
080 1 |a 616  |2 2011 
080 1 |a 577  |2 2011 
100 1 |a Rade, Anamarija  |4 aut 
245 1 0 |a Simple thrombin-based method for eliminating fibrinogen interference in serum protein electrophoresis of haemodialysed patients  |h [Elektronička građa] /  |c Anamarija Rade, Anamarija Đuras, Irena Kocijan, Patricija Banković Radovanović, Ana Turčić. 
300 |b Ilustr., graf. prikazi. 
504 |a Bibliografija: 27 jed. 
504 |a Summary. 
520 |a Introduction: Serum samples of haemodialysed patients collected through vascular access devices, e.g. central venous catheter (CVC) can contain residual heparin, which can cause incomplete clotting and consequently fibrinogen interference in serum protein electrophoresis (SPE). We hypothesized that this problem may be overcome by addition of thrombin and aimed to find a simple thrombin-based method for fibrinogen interference removal. Materials and methods: Blood samples of 51 haemodialysed patients with CVC were drawn through catheter into Clot Activator Tube (CAT) and Rapid Serum Tube Thrombin (RST) vacutainers (Becton Dickinson, New Jersey, USA) following the routine hospital protocols and analysed with gel-electrophoresis (Sebia, Lisses, France). Samples were redrawn in the CAT tubes and re-analysed after being treated with thrombin using two methods: transferring CAT serum into RST vacutainer and treatment of CAT serum with fibrinogen reagent (Multifibren U, Siemens, Marburg, Germany). Results: Direct blood collection in RST proved to be slightly more efficient than CAT in removing the interfering band in beta fraction (CAT removed 6/51 and RST removed 12/51, P = 0.031). Transferring CAT serum into the RST vacutainer proved to be more efficient for subsequent removal of interfering band from CAT serum than the addition of fibrinogen reagent (39/45 vs. 0/45 samples with efficiently removed interfering band, P <0.001). Conclusion: Fibrinogen interference caused by incomplete clotting because of residual heparin can be overcome by addition of thrombin. Transferring CAT serum into the RST vacutainer was the most efficient method. 
653 0 |a Fibrinogen  |a Elektroforeza  |a Trombin  |a Hemodijaliza 
700 1 |a Đuras, Anamarija  |4 aut 
700 1 |a Kocijan, Irena,  |c biokemičarka  |4 aut 
700 1 |a Banković-Radovanović, Patricija  |4 aut 
700 1 |a Turčić, Ana  |4 aut 
773 0 |t Biochemia medica (Online)  |x 1846-7482  |g 30 (2020), 2 ; str. 265-271  |w nsk.(HR-ZaNSK)000655372 
981 |b Be2020  |b B03/20 
998 |b dalo2107 
856 4 0 |u https://doi.org/10.11613/BM.2020.020705 
856 4 0 |u https://www.biochemia-medica.com/en/journal/30/2/10.11613/BM.2020.020705  |y Biochemia medica (Online) 
856 4 1 |y Digitalna.nsk.hr 
856 4 0 |u https://hrcak.srce.hr/238908  |y Hrčak 

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