3D cell technology in biomedical research
Traditional two dimensional cell culture has enabled great strides in biomedicine but needs to be improved to be able to keep up with the demands of modern biomedical research. 2D monolayer culture cannot replicate tissue responses and needs to be supplemented with extensive animal research. Growing...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001104462/Details |
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| Matična publikacija: |
Collegium antropologicum (Online) 44 (2020), 3 ; str. 171-174 |
| Glavni autori: | Mišković Špoljarić, Katarina (Author), Jukić, Marijana, biologinja, Opačak-Bernardi, Teuta, Glavaš-Obrovac, Ljubica |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.5671/ca.44.3.10 Hrčak Collegium antropologicum (Online) |
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| 100 | 1 | |a Mišković Špoljarić, Katarina |4 aut | |
| 245 | 1 | 0 | |a 3D cell technology in biomedical research |h [Elektronička građa] / |c Katarina Mišković Špoljarić, Marijana Jukić, Teuta Opačak-Bernardi, Ljubica Glavaš-Obrovac. |
| 246 | 3 | |a Three-dimensional technology in biomedical research | |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografske bilješke na kraju teksta. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a Traditional two dimensional cell culture has enabled great strides in biomedicine but needs to be improved to be able to keep up with the demands of modern biomedical research. 2D monolayer culture cannot replicate tissue responses and needs to be supplemented with extensive animal research. Growing cells in three dimensional scaffolds provides a more functional model for biomedical research than traditional monolayer culture. Depending on the needs and the complexity of the model there are several ways that 3D models can be initiated. Simple spheroids can be grown in low adherence plates and in hanging drops while larger spheroids and co-cultured ones need to be grown in systems with greater support such as hydro gels. The system that offers the greatest flexibility is the magnetic levitation approach. In the paper we offer a brief resume to various 3D methods and their characteristics to ease the choice of methods for implementing 3D cell culture techniques. | ||
| 520 | |a Klasična dvodimenzionalna stanična kultura omogućila je veliki napredak u biomedicini, ali potrebno ju je unaprijediti kako bi odgovorila zahtjevima modernih biomedicinskih istraživanja. Monoslojna 2D kultura ne može replicirati tkivni odgovor i potrebno ju je nadopuniti iscrpnim istraživanjima na životinjama. Uzgoj stanica u trodimenzionalnim potpornim sustavima rezultira funkcionalnijim biomedicinskim modelima u odnosu na klasične monoslojne kulture. U ovisnosti o potrebama i složenosti istraživanja, 3D modeli se mogu formirati na nekoliko načina. Jednostavni sferoidi se mogu uzgojiti kao viseća kap te na slabo adherirajućim površinama dok se veliki sferoidi i ko-kulture uzgajaju u jačim sustavima podrške poput hidrogelova. Magnetska levitacija je jedan od načina pripreme koji omogućuje najveću fleksibilnost u uzgoju sferoida. U radu ćemo ponuditi kratki pregled različitih 3D modela i njihovih karakteristika kako bismo olakšali odabir metode kod uspostave 3D kulture. | ||
| 653 | 0 | |a Biomedicinska istraživanja |a Stanična kultura |a Stanična proliferacija |a 3D model | |
| 700 | 1 | |a Jukić, Marijana, |c biologinja |4 aut | |
| 700 | 1 | |a Opačak-Bernardi, Teuta |4 aut | |
| 700 | 1 | |a Glavaš-Obrovac, Ljubica |4 aut | |
| 773 | 0 | |t Collegium antropologicum (Online) |x 1848-9486 |g 44 (2020), 3 ; str. 171-174 |w nsk.(HR-ZaNSK)000842419 | |
| 981 | |b Be2020 |b B03/20 | ||
| 998 | |b tino2107 | ||
| 856 | 4 | 0 | |u https://doi.org/10.5671/ca.44.3.10 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/247636 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |
| 856 | 4 | 0 | |u https://www.collantropol.hr/antropo/article/view/1790 |y Collegium antropologicum (Online) |