Optimal surface preparation for wood anatomy research of invasive species by scanning electron microscopy
Research was done to develop the optimal method of wood surface preparation for scanning electron microscopy (SEM). Since 2018, environmental scanning electron microscope (ESEM™) FEI Quanta 250 has been installed at the Department of Wood Science and Technology in Ljubljana. We tested several method...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001108330/Details |
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| Matična publikacija: |
Drvna industrija (Online) 71 (2020), 2 ; str. 117-127 |
| Glavni autori: | Merela, Maks (Author), Thaler, Nejc, Balzano, Angela, Plavčak, Denis |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.5552/drvind.2020.1958 Hrčak |
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| 024 | 7 | |2 doi |a 10.5552/drvind.2020.1958 | |
| 035 | |a (HR-ZaNSK)001108330 | ||
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| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 67/68 |2 2011 | |
| 080 | 1 | |a 630 |2 2011 | |
| 100 | 1 | |a Merela, Maks |4 aut | |
| 245 | 1 | 0 | |a Optimal surface preparation for wood anatomy research of invasive species by scanning electron microscopy |h [Elektronička građa] = |b Optimalna priprema površine drva za istraživanje anatomije invazivnih vrsta drva pretražnim elektronskim mikroskopom / |c Maks Merela, Nejc Thaler, Angela Balzano, Denis Plavčak. |
| 246 | 3 | 1 | |a Optimalna priprema površine drva za istraživanje anatomije invazivnih vrsta drva pretražnim elektronskim mikroskopom |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 47 jed. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a Research was done to develop the optimal method of wood surface preparation for scanning electron microscopy (SEM). Since 2018, environmental scanning electron microscope (ESEM™) FEI Quanta 250 has been installed at the Department of Wood Science and Technology in Ljubljana. We tested several methods for the pre-preparation and cutting of wood surfaces for SEM analyses. The samples had been either dried, soaked in water and frozen, impregnated with paraffin or simply moistened before cutting. We analysed wood surfaces obtained by splitting, sawing, planing, sanding and cutting on a sliding microtome with different blades. The effect of gold coating on the SEM image quality was also evaluated. Best results were obtained by cutting a pre-moistened surface on a sliding microtome with a low profile replaceable blade and gold coated afterwards. Determined methodology is technically less demanding, not time consuming and obtains results that satisfy needs for wood anatomy research at magnifications up to 12.000x. Guidelines for the optimal preparation of samples were prepared, and theoretical and practical basis for investigations of wood anatomy using SEM were provided. The method was afterwards used in analyses of invasive alien plant species – investigating their anatomical structure in the framework of the AlienPLAntSpEcies - APPLAUSE project (Urban Innovative Actions initiative). It was demonstrated that the use of the SEM opened new scope in detailed investigations of the wood structure and properties. | ||
| 520 | |a Istraživanje je provedeno kako bi se razvila optimalna metoda pripreme drvne površine za pretražnu elektronsku mikroskopiju (SEM). Od 2018. na Odsjeku za znanost i tehnologiju o drvu u Ljubljani instaliran je uređaj za pretražnu elektronsku mikroskopiju u uvjetima okoliša (ESEMTM) FEI Quanta 250. Proučavali smo nekoliko metoda za pripremu i rezanje površine drva za SEM analizu. Uzorci su sušeni, potapani u vodi i smrzavani te prije rezanja impregnirani parafi nom ili samo navlaženi. Analizirali smo površine drva dobivene cijepanjem, piljenjem, blanjanjem, brušenjem i rezanjem na mikrotomu različitim oštricama. Također je proučavan utjecaj zlatnog premaza na kvalitetu SEM fotografi ja. Najbolji rezultati dobiveni su rezanjem prethodno navlažene površine na mikrotomu, i to zamjenjivim noževima niskog profi la, nakon čega je površina drva pozlaćena. Primijenjena je metodologija tehnički manje zahtjevna, ne oduzima mnogo vremena, a dobiveni rezultati zadovoljavaju potrebe istraživanja anatomije drva pri povećanju i do 12 000 puta. Sastavljene su smjernice za optimalnu pripremu uzoraka te je postavljena teorijska i praktična osnova za istraživanje anatomije drva primjenom SEM-a. Metoda je potom primijenjena za istraživanje invazivnih biljnih vrsta – za ispitivanje njihove anatomske strukture u sklopu projekta AlienPLAntSpEcies – APPLAUSE (Urban Innovative Actions). Potvrđeno je da je upotreba SEM-a otvorila novo područje u detaljnim istraživanjima strukture i svojstava drva. | ||
| 653 | 0 | |a Anatomija drva |a Invazivne vrste |a Pretražna elektronska mikroskopija | |
| 700 | 1 | |a Thaler, Nejc |4 aut | |
| 700 | 1 | |a Balzano, Angela |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Plavčak, Denis |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Drvna industrija (Online) |x 1847-1153 |g 71 (2020), 2 ; str. 117-127 |w nsk.(HR-ZaNSK)000688399 | |
| 981 | |b Be2020 |b B03/20 | ||
| 998 | |b tino2109 | ||
| 856 | 4 | 0 | |u https://doi.org/10.5552/drvind.2020.1958 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/238920 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |