Comparison of the novel Uroquattro HB<M system and classical phenotypic method for rapid screening of multidrug-resistant organism colonization at the University Hospital Centre Split, Croatia
Background. Infections caused by multidrug-resistant organisms (MDRO) are difficult to treat and associated with poor outcomes for patients. Therefore, early identification and management of colonization are essential as first steps in infection prevention. Culture-based methods have been widely use...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001116928/Details |
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| Matična publikacija: |
Infektološki glasnik (Online) 40 (2020), 1 ; str. 15-19 |
| Glavni autori: | Marinović, Jelena (Author), Novak, Anita, liječnica, Rubić, Žana, Goić-Barišić, Ivana, Radić, Marina, liječnica, Barišić, Miroslav, stomatolog, Tonkić, Marija |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.37797/ig.40.1.3 Hrčak |
| LEADER | 05622naa a22004214i 4500 | ||
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| 008 | 211028s2020 ci |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.37797/ig.40.1.3 | |
| 035 | |a (HR-ZaNSK)001116928 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b eng |b hrv | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 616 |2 2011 | |
| 100 | 1 | |a Marinović, Jelena |4 aut | |
| 245 | 1 | 0 | |a Comparison of the novel Uroquattro HB&L™ system and classical phenotypic method for rapid screening of multidrug-resistant organism colonization at the University Hospital Centre Split, Croatia |h [Elektronička građa] = |b Usporedba nove brze metode Uroquattro HB&L™ s klasičnom fenotipskom metodom probira kliconoštva višestruko-otpornim organizmima u Kliničkom bolničkom centru Split, Hrvatska / |c Jelena Marinović, Anita Novak, Žana Rubić, Ivana Goić-Barišić, Marina Radić, Miroslav Barišić, Marija Tonkić. |
| 246 | 3 | 1 | |a Usporedba nove brze metode Uroquattro HB&L™ s klasičnom fenotipskom metodom probira kliconoštva višestruko-otpornim organizmima u Kliničkom bolničkom centru Split, Hrvatska |
| 504 | |a Bibliografija: 20 jed. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a Background. Infections caused by multidrug-resistant organisms (MDRO) are difficult to treat and associated with poor outcomes for patients. Therefore, early identification and management of colonization are essential as first steps in infection prevention. Culture-based methods have been widely used for MDRO screening. The turnaround time (TAT) for the identification of carriers varies between 48-72 h with this method. The aim of our study was to compare the performance of the new rapid semiautomatic method for detection of MDRO (HB&L Uroquattro, Alifax) with standard cultivation on selective media. Methods. Twenty-one axillary, 20 nose and 19 rectal swabs were taken in duplicate on two selected days at the University Hospital Centre Split, Croatia. Swabs were cultivated and MDRO isolates were identified on selective media (Chromagar MRSA and Chromagar ESBL) according to the standard operating procedure. Novel Alifax method was performed according to manufacturer’s instructions. Results. TAT for the new method was significantly lower (6.5 h) in comparison to the classical method. With classical method, 10 extended spectrum ß-lactamases (ESBL) producing strains from 10 different rectal specimens were isolated on Chromagar ESBL media. Exactly the same specimens were positive for the presence of ESBL-producing bacteria by rapid Alifax method. On selective Chromagar MRSA media, 11 MDRO were isolated, while rapid method detected 11 MDRO from the same specimens. Conclusions. High concordance of positive and negative results obtained with classical and rapid method is encouraging. However, our study was performed on a small sample size and further research with larger sample size is needed. | ||
| 520 | |a Uvod: Infekcije uzrokovane višestruko otpornim organizmima (MDRO) se teško liječe i često imaju nepovoljan ishod za bolesnika. Budući da infekciji najčešće prethodi kolonizacija, rana identifikacija koloniziranih bolesnika je neophodna u prevenciji nastanka infekcije. Za probir MDRO najčešće se koriste kultivacijske metode kojima vrijeme potrebno za izdavanje nalaza (TAT) iznosi od 48 do 72 h. Cilj ove studije bio je usporediti rezultate nove, brze, poluautomatske metode za detekciju MDRO (HB&L Uroquattro, Alifax) sa standardnom kultivacijom na selektivnim podlogama. Metode/materijali: Tijekom dva nasumično odabrana dana, bolesnicima hospitaliziranima u Kliničkom bolničkom centru Split, uzorkovano je ukupno 21 aksilarnih, 19 rektalnih te 20 briseva vestibuluma nosa. Svaki obrisak je prikupljen u duplikatu, jedan za kultivacijsku, a drugi za brzu poluatomatsku metodu. Brisevi su kultivirani i MDRO izolati identificirani na selektivnim podlogama (Chromagar MRSA i Chromagar ESBL) prema standardnom laboratorijskom protokolu. Nova Alifaxova metoda je provedena prema uputama proizvođača. Rezultati: U usporedbi s klasičnom metodom, TAT nove metode je bio značajno kraći (6,5 h). Klasičnom metodom je na Chromagar ESBL selektivnom mediju izolirano 10 sojeva koji stvaraju ß-laktamaze proširenog spektra (ESBL) iz 10 različitih rektalnih uzoraka. Isti uzorci su bili pozitivni na prisustvo ESBL- producirajućih bakterija brzom Alifaxovom metodom. Kultivacijom na selektivnom Chromagar MRSA mediju i brzom metodom otkriveno je 11 uzoraka pozitivnih na prisustvo MDRO. Zaključak: Visoki stupanj podudarnosti otkrivanja pozitivnih i negativnih uzoraka dobiven klasičnom i novom metodom, kao i značajno skraćivanje TAT-a novom metodom je ohrabrujući. Budući je studija provedena na relativno malom uzorku, potrebno je istraživanje proširiti na veći broj ispitanika. | ||
| 653 | 0 | |a Bakterijske infekcije |a MDR bakterije |a Staphylococcus aureus |a Beta-laktamaze |a Lasersko liječenje | |
| 700 | 1 | |a Novak, Anita, |c liječnica |4 aut | |
| 700 | 1 | |a Rubić, Žana |4 aut | |
| 700 | 1 | |a Goić-Barišić, Ivana |4 aut | |
| 700 | 1 | |a Radić, Marina, |c liječnica |4 aut | |
| 700 | 1 | |a Barišić, Miroslav, |c stomatolog |4 aut | |
| 700 | 1 | |a Tonkić, Marija |4 aut | |
| 773 | 0 | |t Infektološki glasnik (Online) |x 1848-7769 |g 40 (2020), 1 ; str. 15-19 |w nsk.(HR-ZaNSK)000829777 | |
| 981 | |b Be2020 |b B04/20 | ||
| 998 | |b tino2111 | ||
| 856 | 4 | 0 | |u https://doi.org/10.37797/ig.40.1.3 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/243759 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |