miR-7 and miR-34a sequence cloning and expression in a1235 glioblastoma cell line
miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids conta...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001122397/Details |
|---|---|
| Matična publikacija: |
Molecular and experimental biology in medicine 3 (2020), 2 ; str. 31-38 |
| Glavni autori: | Kolić, Dora (Author), Horvat, Luka, molekularni biolog, Šetinc, Maja, Antica, Mariastefania, Matulić, Maja |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.33602/mebm.3.2.4 Molecular and experimental biology in medicine Hrčak |
| LEADER | 02314naa a22004094i 4500 | ||
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| 003 | HR-ZaNSK | ||
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| 007 | cr|||||||||||| | ||
| 008 | 211228s2020 ci a |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.33602/mebm.3.2.4 | |
| 035 | |a (HR-ZaNSK)001122397 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 575 |2 2011 | |
| 100 | 1 | |a Kolić, Dora |4 aut | |
| 245 | 1 | 0 | |a miR-7 and miR-34a sequence cloning and expression in a1235 glioblastoma cell line |h [Elektronička građa] / |c Dora Kolic, Luka Horvat, Maja Setinc, Mariastefania Antica, Maja Matulic. |
| 246 | 3 | |a MicroRNA-7 and miR-34a sequence cloning | |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 34 jed. | ||
| 504 | |a Abstract. | ||
| 520 | |a miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents. | ||
| 653 | 0 | |a MikroRNA |a Stanične linije glioblastoma |a Tumor-supresorski gen | |
| 700 | 1 | |a Horvat, Luka, |c molekularni biolog |4 aut | |
| 700 | 1 | |a Šetinc, Maja |4 aut | |
| 700 | 1 | |a Antica, Mariastefania |4 aut | |
| 700 | 1 | |a Matulić, Maja |4 aut | |
| 773 | 0 | |t Molecular and experimental biology in medicine |x 2584-671X |g 3 (2020), 2 ; str. 31-38 |w nsk.(HR-ZaNSK)000985906 | |
| 981 | |b Be2020 |b B04/20 | ||
| 998 | |b tino2202 | ||
| 856 | 4 | 0 | |u https://doi.org/10.33602/mebm.3.2.4 |
| 856 | 4 | 0 | |u https://mebm.eu/index.php/journal/article/view/76 |y Molecular and experimental biology in medicine |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/261476 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |