Molecular investigations on the Rajanpuri strain of Beetal goat breed of Pakistan by complete mtDNA displacement region and microsatellite markers
The mitochondrial DNA sequences of DNA-D loops (mtDNA) from 105 individuals (11 Beetal goat Rajanpuri strains and 94 sequences extracted from GenBank) from 19 geographically and phenotypically defined domestic goat breeds in Pakistan were analysed in this experiment. In this study, we examined varia...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001145218/Details |
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| Matična publikacija: |
Veterinarska stanica (Online) 52 (2021), 5 ; str. 511-523 |
| Glavni autori: | Wajid, Abdul (Author), Hussain, Tanveer, Marikar, Faiz M. M. T., Musthafa, Muneeb M., Abbas, Kamran, Ali, Akhtar, Babar, Masroor Ellahi |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.46419/vs.52.5.8 Hrčak |
| LEADER | 04984naa a22004214i 4500 | ||
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| 001 | NSK01001145218 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20221121130640.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 220803s2021 ci ad |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.46419/vs.52.5.8 | |
| 035 | |a (HR-ZaNSK)001145218 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 636.09 |2 2011 | |
| 100 | 1 | |a Wajid, Abdul |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Molecular investigations on the Rajanpuri strain of Beetal goat breed of Pakistan by complete mtDNA displacement region and microsatellite markers |h [Elektronička građa] / |c Abdul Wajid, Tanveer Hussain, Faiz M. M. T. Marikar, Muneeb M. Musthafa, Kamran Abbas, Akhtar Ali, Masroor Ellahi Babar. |
| 300 | |b Ilustr. ; graf. prikazi. | ||
| 504 | |a Bibliografija: 31 jed. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a The mitochondrial DNA sequences of DNA-D loops (mtDNA) from 105 individuals (11 Beetal goat Rajanpuri strains and 94 sequences extracted from GenBank) from 19 geographically and phenotypically defined domestic goat breeds in Pakistan were analysed in this experiment. In this study, we examined variability and molecular phylogeny of breeds. A total of 81 haplotypes were observed in 105 individuals, with a haplotype diversity of 0.984±0.006 and nucleotide diversity of 0.03953±0.00843. Phylogenetic analysis based on the mtDNA hyper variable segment (HVI) of the control region (481 bp), showed four mtDNA haplogroups (A, B1, C, and D) identified in Pakistani domestic goats, in which haplogroup A (84.11%) was dominant and widely distributed among all investigated breeds. The study revealed that all Rajanpuri strain haplotypes belonged to haplogroup A. The Rajanpuri is a rare local strain of Beetal goat breed located in western Punjab province of Pakistan. The results of genetic diversity based on 11 microsatellite loci revealed allelic diversity (3.6363) and high genetic diversity (0.8342) in the examined Rajanpuri goat breeds. The analysis for signature bottleneck events, using three models, revealed significant deviation of Rajanpuri goats from mutation drift equilibrium. The qualitative test of mode shift analysis also supported the results obtained under three models, indicating the presence of a recent genetic bottleneck in the Rajanpuri strain. This study provides the first information on the mtDNA architecture, genetic diversity and bottleneck analysis, which will be useful in the conservation and management of the highly valued Rajanpuri goat. | ||
| 520 | |a Mitohondrijske DNK sekvence DNK-D petlji (mtDNK) 105 jedinki (11 ratalpuri sojeva beetal koze i 94 sekvence iz GenBank) od 19 geografskih i fenotipičnih domaćih pasmina koza u Pakistanu rabljeno je u ovom eksperimentu. U ovoj smo studiji istražili varijabilnost i njezinu molekularnu filogeniju. Zamijećen je ukupno 81 različit haplotip u 105 jedinki s raznolikošću haplotipova 0,984±0,006 i nukleotidnom raznolikošću 0,03953±0,00843. Filogenetskom analizom ustvrđen iz mtDNK hipervarijabilni segment (HVI) kontrolnog područja (481 bp) i pokazala je četiri haploskupine mtDNK (A, B1, C i D) identificirane u pakistanskih domaćih koza, u kojima se haploskupina A (84,11 %) pokazala dominantnom i široko rasprostranjenom među svim istraživanim pasminama. Studija je otkrila da svi haplotipovi rajanpuri soja pripadaju haploskupini A. Rajanpuri je rijedak lokalni soj beetal pasmine koza smješten u zapadnoj regiji provincije Punjab u Pakistanu. Rezultati genetske raznolikosti na temelju 11 mikrosatelitnih lokusa otkrili su alelnu raznolikost (3,6363) i visoku genetsku raznolikost (0,8342) istraživane rajanpuri pasmine koza. Rezultati analize za potpis za događaje uskog grla uporabom tri modela otkrili su značajno odstupanje rajanpuri koza od ravnoteže mutacija i pomaka. Kvalitativni test analize modalnog pomaka podržao je i rezultate dobivene trima modelima, ukazujući na prisutnost genetskog uskog grla u rajanpuri soja u nedavnoj prošlosti. Ova studija po prvi put pruža informacije o arhitekturi mtDNK, genetskoj raznolikosti, analizi uskog grla koje će biti korisne u donošenju odluke o očuvanju i upravljanju cijenjenom i skupocjenom rajanpuri kozom. | ||
| 653 | 0 | |a mtDNA |a Mikrosatelitni marker |a Filogenetska analiza |a Rajanpuri koza | |
| 700 | 1 | |a Hussain, Tanveer |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Marikar, Faiz M. M. T. |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Musthafa, Muneeb M. |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Abbas, Kamran |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Ali, Akhtar |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Babar, Masroor Ellahi |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Veterinarska stanica (Online) |x 1849-1170 |g 52 (2021), 5 ; str. 511-523 |w nsk.(HR-ZaNSK)000855872 | |
| 981 | |b Be2021 |b B03/21 | ||
| 998 | |b tino2211 | ||
| 856 | 4 | 0 | |u https://doi.org/10.46419/vs.52.5.8 |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/248800 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |