Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics
Equex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation. However, Equex paste produced by Nova Chemical Sales Inc. (MA, USA) is not currently available. The aim of the present study was to determine the optimal con...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001145243/Details |
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| Matična publikacija: |
Veterinarska stanica (Online) 52 (2021), 3 ; str. 285-295 |
| Glavni autori: | Suwimonteerabutr, Junpen (Author), Nuntapaitoon, Morakot, Tummaruk, Padet |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.46419/vs.52.3.8 Hrčak |
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| 024 | 7 | |2 doi |a 10.46419/vs.52.3.8 | |
| 035 | |a (HR-ZaNSK)001145243 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 636.09 |2 2011 | |
| 100 | 1 | |a Suwimonteerabutr, Junpen |4 aut |9 HR-ZaNSK | |
| 245 | 1 | 0 | |a Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics |h [Elektronička građa] / |c Junpen Suwimonteerabutr, Morakot Nuntapaitoon, Padet Tummaruk. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 47 jed. | ||
| 504 | |a Abstract ; Sažetak. | ||
| 520 | |a Equex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation. However, Equex paste produced by Nova Chemical Sales Inc. (MA, USA) is not currently available. The aim of the present study was to determine the optimal concentration of Minitube Equex paste (Minitube, Tiefenbach, Germany) for boar semen cryopreservation in comparison with Nova Equex STM paste (control). Fifteen ejaculates from 12 mature boars were collected by the glove-hand method. Each ejaculate was aliquoted and cryopreserved in base freezing extender III as Tris-citrate egg yolk (TEY) extender plus 9.0% glycerol classified into four groups. Group I was the control and included only 1.5% Nova Equex STM paste. Groups II, III and IV were the experiment groups, and contained different concentrations of Minitube Equex paste (Group II: 1.5%; Group III: 1.7%; and Group IV: 1.9%) added to the freezing extender III. After freezing and thawing, sperm motility characteristics were evaluated by Sperm Class Analyzer® incubated at 37 °C for 0 (10 min), 1 and 2 h post-thawing. In Group IV after thawing at 0 h, rapid velocity and the velocity curved line were significantly higher than in Groups II and III (P <0.05) but did not differ from Group I. Moreover, after thawing at 1 h, LIN (linearity) in Group IV was higher than in Group II (P <0.05), but did not differ from the other groups. In conclusion, the most suitable concentration of Minitube Equex paste in the current protocol was 1.9% supplemented with 9.0% glycerol in TEY-based freezing extender III, based on the conformity between data from manual guides and the observed sperm motility characteristics results. | ||
| 520 | |a Equex pasta je neprožimajuće kriozaštitno sredstvo (CPA) za koje je dobro poznato da poboljšava preživljavanje spermija nakon odmrzavanja tijekom krioprezervacije sjemena nerasta. Međutim, Equex pasta koju proizvodi tvrtka Nova Chemical Sales Inc. (MA, SAD) trenutno nije dostupna na tržištu. Cilj ove studije bio je odrediti optimalnu koncentraciju Minitube Equex paste (Minitube, Tiefenbach, Njemačka) za krioprezervaciju sjemena nerasta u usporedbi s Nova Equex STM pastom (kontrola). Prikupljeno je petnaest ejakulata 12 zrelih nerasta metodom manualne fiksacije penisa. Svaki ejakulat bio je raspodijeljen i krioprezerviran u osnovnom razrjeđivaču za duboko zamrzavanje III – razrjeđivač trinatrijev citrat s dodatkom žutanjka jajeta (TEY) plus 9,0 % glicerola, razvrstano u četiri skupine (tj. skupine I, II, III i IV). Skupina I: 1,5 % Nova Equex STM pasta (kontrola) i tri različite koncentracije Minitube Equex paste (Skupina II: 1,5 %; Skupina III: 1,7 %; i Skupina IV: 1,9 %) dodano je osnovnom razrjeđivaču za duboko zamrzavanje III. Nakon zamrzavanja i odmrzavanja, karakteristike pokretljivosti spermija procijenjene su pomoću analizatora sperme Sperm Class Analyzer® inkubirano na 37 °C, 0 (10 min), 1 i 2 h nakon odmrzavanja. U Skupini IV, 0 h nakon odmrzavanja, brzina i krivulja brzine bile su značajno više nego u Skupinama II i III (P <0,05), ali se nisu razlikovale od Skupine I. Nadalje, 1 h nakon odmrzavanja, LIN (linearnost) u Skupini IV bila je veća nego u Skupini II (P <0,05), ali se nije razlikovala od ostalih skupina. Zaključno, najprikladnija koncentracija Minitube Equex paste u ovom protokolu bila je 1,9 % uz dodatak 9,0 % glicerola u TEY razrjeđivaču za zamrzavanje III, zbog sukladnosti između podataka iz priručnika i zamijećenih rezultata karakteristika pokretljivosti spermija. | ||
| 653 | 0 | |a Nerast |a Sjeme |a Krioprezervacija |a Pokretljivost spermija | |
| 700 | 1 | |a Nuntapaitoon, Morakot |4 aut |9 HR-ZaNSK | |
| 700 | 1 | |a Tummaruk, Padet |4 aut |9 HR-ZaNSK | |
| 773 | 0 | |t Veterinarska stanica (Online) |x 1849-1170 |g 52 (2021), 3 ; str. 285-295 |w nsk.(HR-ZaNSK)000855872 | |
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