Practical application of the progesterone P4 rapid test in cow's milk for early pregnancy detection

Pregnancy diagnosis is an essential part of fertility management in cattle. Early detection of pregnancy 21 days after artificial insemination (AI) allows for early recognition and rebreeding of non-pregnant animals with a minimum delay, which benefits the economic interest for farmers. Rapid proges...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01001145248/Details
Matična publikacija: Veterinarska stanica (Online)
52 (2021), 3 ; str. 323-329
Glavni autori: Mehmedi, Blerta (Author), Gecaj, Rreze M., Kastrati, Taulant, Heltai, Miklós, Turmalaj, Luigj
Vrsta građe: e-članak
Jezik: eng
Predmet:
Online pristup: https://doi.org/10.46419/vs.52.3.3
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100 1 |a Mehmedi, Blerta  |4 aut 
245 1 0 |a Practical application of the progesterone P4 rapid test in cow's milk for early pregnancy detection  |h [Elektronička građa] /  |c Blerta Mehmedi, Rreze M. Gecaj, Taulant Kastrati, Miklós Heltai, Luigj Turmalaj. 
300 |b Graf. prikazi. 
504 |a Bibliografija: 35 jed. 
504 |a Abstract ; Sažetak. 
520 |a Pregnancy diagnosis is an essential part of fertility management in cattle. Early detection of pregnancy 21 days after artificial insemination (AI) allows for early recognition and rebreeding of non-pregnant animals with a minimum delay, which benefits the economic interest for farmers. Rapid progesterone P4 tests are widely used in fertility management strategies, yet there is limited data about the efficiency of tests for P4 measurements in cow"s milk. By measuring P4 concentrations in the milk of different breeds: Red Holstein, Black-Holstein, Simmental, and local crossbreeds, this study aimed to assess the practical application and efficiency of an early and rapid pregnancy detection test from cow"s milk. The study was conducted in 2017–2018 and milk samples were taken from animals at day 21 after artificial insemination (AI) to diagnose pregnancy. The test indicated that of the total cows (n=400) included in this study, 69% were pregnant and 31% were not pregnant, as interpreted based on the P4 Rapid (Ridgeway Science UK) test results. Accuracy of the early diagnosis was verified 60 days after AI (without further AI of cows) by rectal palpation. Based on this examination, 263 (66%) cows were diagnosed as pregnant, while 14 (34%) were diagnosed as non-pregnant. The highest accuracy in pregnancy detection was found in the local crossbreeds and the Simmental breed, with 97% correct diagnosis of cows, followed by Red Holstein with 94% and Black Holstein with an accuracy of 90%. This study showed that the use of progesterone P4 rapid tests in milk samples is an effective, fast and accurate method for the early detection of pregnancy in cows, with an accuracy rate of 90 to 97% based on cattle breed. 
520 |a Dijagnoza gravidnosti bitan je dio menadžmenta plodnosti u goveda. Rana dijagnostika gravidnosti 21 dana nakon osjemenjavanja omogućava rano prepoznavanje i ponovno umjetno osjemenjivanje (UO) negravidnih krava s minimalnim produžetkom servisnog razdoblja te je od ekonomskog interesa za poljoprivrednike. Brzi progesteronski P4 testovi puno se koriste u strategijama upravljanja plodnosti, ali postoji ograničen broj podataka o učinkovitosti tih testova za mjerenje P4 iz kravljeg mlijeka. Mjereći koncentraciju progesterona P4 iz mlijeka različitih pasmina krava kao što su: crveni holstein, crni holstein, simentalska pasmina i lokalne križane pasmine, ova studija ima za cilj procjenu praktične primjene i učinkovitosti ranog i brzog testa dijagnostike gravidnosti iz kravljeg mlijeka. Studija je provedena u razdoblju od 2017. i 2018., a uzorci mlijeka uzorkovani su životinjama 21. dana nakon UO u svrhu dijagnostike gravidnosti. Od 400 krava uključenih u ovo istraživanje, interpretacijom rezultata na temelju uporabe progesteronskog P4 brzog testa (Ridgeway Science, VB) 69 % testiranih krava je bilo gravidno, dok 31 % krava nisu bile gravidne. Točnost rane dijagnoze potvrđena je 60 dana nakon UO krava (bez daljnjih UO krava) rektalnom palpacijom. Na temelju ovog ispitivanja 263 krave, odnosno njih 66 % je dijagnosticirano gravidnima, dok 14 krava ili 34 % nisu bile gravidne. Najveća preciznost u otkrivanju gravidnosti ovom metodom ustvrđena je u lokalnih križanih pasmina i krava simentalske pasmine s 97 % točno dijagnosticiranih krava, a slijede ih pasmina crveni holstein s 94 % i crni holstein s 90 % točnosti. Naše istraživanje pokazalo je da se s preciznošću od 90 % do 97 % za različite testirane pasmine krava uporaba brzih progesteronskih P4 testova u uzorcima mlijeka pokazala učinkovitom, brzom i točnom metodom za rano otkrivanje gravidnosti krava. 
653 0 |a Kravlje mlijeko  |a Umjetno osjemenjivanje  |a Gravidnost  |a Screening test  |a Rektalna pretraga 
700 1 |a Gecaj, Rreze M.  |4 aut  |9 HR-ZaNSK 
700 1 |a Kastrati, Taulant  |4 aut  |9 HR-ZaNSK 
700 1 |a Heltai, Miklós  |4 aut  |9 HR-ZaNSK 
700 1 |a Turmalaj, Luigj  |4 aut 
773 0 |t Veterinarska stanica (Online)  |x 1849-1170  |g 52 (2021), 3 ; str. 323-329  |w nsk.(HR-ZaNSK)000855872 
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