Enhanced production of alkaline protease by Teredinobacter turnirae in fed-batch feremntation using a concentrated medium
To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity ob...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01000716495/Details |
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| Matična publikacija: |
Chemical and biochemical engineering quarterly 22 (2008), 1 ; str. 119-124, 139 |
| Glavni autor: | Beshay, Usama (-) |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
CABEQ |
| LEADER | 01801caa a2200289 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000716495 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20120906115151.0 | ||
| 007 | ta | ||
| 008 | 091019s2008 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)719064 | ||
| 035 | |a (HR-ZaNSK)000716495 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv | |
| 042 | |a croatica | ||
| 080 | |a 66 |2 MRF 1998. | ||
| 100 | 1 | |a Beshay, Usama | |
| 245 | 1 | 0 | |a Enhanced production of alkaline protease by Teredinobacter turnirae in fed-batch feremntation using a concentrated medium / |c Usama Beshay. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 29 jed. | ||
| 504 | |a Sažetak | ||
| 520 | 8 | |a To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity obtained was 8500 U mL–1 compared to 3000 U mL–1 obtained by growing Teredinobacter turnirae cells with standard PB-medium in batch culture. For further increase of process efficiency, a high density fed-batch process was developed, where concentrated medium is fed to the cells. Fed-batch process using concentrated medium was evaluated for its ability to improve cell growth and protease titer of T. turnirae. It was found to result in up to 3.9-fold increase in final protease activity compared to batch culture control | |
| 653 | 0 | |a Teredinobacter turnirae |a Alkalna proteaza | |
| 773 | 0 | |t Chemical and biochemical engineering quarterly |x 0352-9568 |g 22 (2008), 1 ; str. 119-124, 139 |w nsk.(HR-ZaNSK)000004762 | |
| 981 | |b B08/08 |p CRO | ||
| 998 | |a rado091019 |c vol2o120906 | ||
| 856 | 4 | 2 | |u http://www.fkit.hr/cabeq/index.html |y CABEQ |