Tolerance of immobilized yeast cells in imidazolium-based ionic liquids

Summary: Ionic liquids (ILs) are considered as another ’green solvent’, after the supercritical carbon dioxide. They are a promising reaction medium for biocatalysis process. The tolerance of active cells in hydrophobic imidazolium-based ILs (1-R-3-methylimidazolium hexafluorophosphate, [RMim][PF6])...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01000735873/Details
Matična publikacija: Food technology and biotechnology
47 (2009), 1 ; str. 62-66
Glavni autor: Yang, Zhong-Hua (-)
Ostali autori: Wang, Yu, kemijski tehnolog (-), Li, Xian-Ke, Lv, Zao-Sheng, Lai, Bin, Yang, Shu-Qin, Liao, Jian-Guo
Vrsta građe: Članak
Jezik: eng
Predmet:
Online pristup: Food Technology and Biotechnology
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035 |a (HR-ZaNSK)000735873 
040 |a HR-ZaNSK  |b hrv  |c HR-ZaNSK  |e ppiak 
042 |a croatica 
080 |a 66  |2 MRF 1998. 
100 1 |a Yang, Zhong-Hua 
245 1 0 |a Tolerance of immobilized yeast cells in imidazolium-based ionic liquids /  |c Zhong-Hua Yang, Rong Zeng, Yu Wang, Xian-Ke Li, Zao-Sheng Lv, Bin Lai, Shu-Qin Yang, Jian-Guo Liao. 
300 |b Ilustr. 
504 |a Bibliografija: 29 jed. 
520 8 |a Summary: Ionic liquids (ILs) are considered as another ’green solvent’, after the supercritical carbon dioxide. They are a promising reaction medium for biocatalysis process. The tolerance of active cells in hydrophobic imidazolium-based ILs (1-R-3-methylimidazolium hexafluorophosphate, [RMim][PF6]) has been studied in this work. Calcium-alginate-entrapped baker’s yeast has been chosen as the model of living cells. The results show that this kind of ILs possess a certain degree of biocompatibility. The tolerance of yeast cells to the ILs decreases with the increase of the R chain length of these ILs. The experiment indicated that 1-butyl-3-methylimidazolium hexafluorophosphate ([BMim][PF6]) possessed excellent biocompatibility compared to the other imidazolium-based ILs. The moisture content in the ILs was the key factor that affected the tolerance. The activity retention of yeast cells pretreated with [BMim][PF6] saturated with water and aqueous [BMim][PF6] biphasic system was about 70 %, but it was only 50 % with the anhydrous [BMim][PF6]. Although the yeast cells were pretreated with [BMim][PF6] for 24 h, the activity retention was up to 45 %. The yeast cells had around 50 % activity after being pretreated 4 times with [BMim][PF6]. This shows that the water immiscible ILs possess good biocompatibility, and they are suitable for application as the reaction medium catalyzed by living cells 
653 0 |a Kvasac  |a Ionska kapljevina  |a Zelena otapala  |a Imidazol  |a Biokompatibilnost 
700 1 |a Wang, Yu,  |c kemijski tehnolog 
700 1 |a Li, Xian-Ke 
700 1 |a Lv, Zao-Sheng 
700 1 |a Lai, Bin 
700 1 |a Yang, Shu-Qin 
700 1 |a Liao, Jian-Guo 
773 0 |t Food technology and biotechnology  |x 1330-9862  |g 47 (2009), 1 ; str. 62-66  |w nsk.(HR-ZaNSK)000174173 
981 |b B03/09 
998 |a Luko100506  |c vol2o121016  |b mili1809 
856 4 2 |u http://hrcak.srce.hr/ftb  |y Food Technology and Biotechnology