Clenbuterol Residues in Plasma and Urine Samples of Food-Producing Pigs During and After Subchronic Exposure to a Growth-Promoting Dose
Summary: The aim of the study is to evaluate the suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screeni...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01000735905/Details |
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| Matična publikacija: |
Food technology and biotechnology 47 (2009), 1 ; str. 67-74 |
| Glavni autor: | Pleadin, Jelka (-) |
| Ostali autori: | Babić-Gojmerac, Tihomira (-), Bratoš, Igor, Lipej, Zoran, Novosel, Dinko, Vulić, Ana |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Food Technology and Biotechnology |
| LEADER | 02615caa a2200325 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000735905 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20121016115144.0 | ||
| 007 | ta | ||
| 008 | 100506s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)738529 | ||
| 035 | |a (HR-ZaNSK)000735905 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 664 |2 MRF 1998. | ||
| 100 | 1 | |a Pleadin, Jelka | |
| 245 | 1 | 0 | |a Clenbuterol Residues in Plasma and Urine Samples of Food-Producing Pigs During and After Subchronic Exposure to a Growth-Promoting Dose / |c Jelka Pleadin, Tihomira Gojmerac, Igor Bratoš, Zoran Lipej, Dinko Novosel, Ana Vulić. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 21 jed | ||
| 520 | 8 | |a Summary: The aim of the study is to evaluate the suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmation method. A high correlation coefficient between these analytical methods was obtained for both urine (R=0.9800) and plasma (R=0.9970) concentrations. Study results show the plasma and urine concentration to vary greatly during oral treatment with clenbuterol for 28 days. The peak urine concentration ((88.54±50.54) ng/mL) recorded on day 21 was 40-fold peak plasma concentration ((2.25±1.54) ng/mL). After withdrawal period, the peak urine clenbuterol concentration ((42.93±10.52) ng/mL) recorded on day 0 was 24-fold plasma concentration ((1.79±0.97) ng/mL). The maximum allowed concentration of 0.5 ng/g in the liver as a regulated matrix for control of clenbuterol abuse was achieved in plasma on day 3 ((0.52±0.26) ng/mL) and in urine on day 7 of treatment withdrawal ((0.45±0.11) ng/mL). Study results indicate that urine and plasma may be suitable matrices for the control of clenbuterol abuse during fattening of food-producing pigs but have a limited value because of the rapidly decreasing concentration upon treatment withdrawal, in plasma in particular | |
| 653 | 0 | |a Raktopamin |a Klenbuterol |a Svinja | |
| 700 | 1 | |a Babić-Gojmerac, Tihomira | |
| 700 | 1 | |a Bratoš, Igor | |
| 700 | 1 | |a Lipej, Zoran | |
| 700 | 1 | |a Novosel, Dinko | |
| 700 | 1 | |a Vulić, Ana | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 47 (2009), 1 ; str. 67-74 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100506 |c vol2o121016 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/ftb |y Food Technology and Biotechnology |