Modelling of continuous L-malic acid production by porcine heart fumarase and fumarase in yeast cells
Summary: Continuous production of L-malic acid will be presented in this paper. The fumarase isolated from porcine heart, fumarase in the permeabilized non-growing cells of baker’s yeast and Saccharomyces bayanus (UVAFERM BC) were used as biocatalysts. In the production of L-malic acid with fumarase...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01000736561/Details |
|---|---|
| Matična publikacija: |
Chemical and biochemical engineering quarterly 23 (2009), 4 ; str. 519-525 |
| Glavni autor: | Vrsalović Presečki, Ana (-) |
| Ostali autori: | Zelić, Bruno (-), Vasić-Rački, Đurđa |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Chemical and Biochemical Engineering Quarterly |
| LEADER | 02157caa a2200289 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000736561 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20180410171639.0 | ||
| 007 | ta | ||
| 008 | 100513s2009 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)739185 | ||
| 035 | |a (HR-ZaNSK)000736561 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 663 |2 MRF 1998. | ||
| 100 | 1 | |a Vrsalović Presečki, Ana | |
| 245 | 1 | 0 | |a Modelling of continuous L-malic acid production by porcine heart fumarase and fumarase in yeast cells / |c A. Vrsalović Presečki, B. Zelić, Đ. Vasić-Rački. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 23 jed. | ||
| 520 | 8 | |a Summary: Continuous production of L-malic acid will be presented in this paper. The fumarase isolated from porcine heart, fumarase in the permeabilized non-growing cells of baker’s yeast and Saccharomyces bayanus (UVAFERM BC) were used as biocatalysts. In the production of L-malic acid with fumarase isolated from porcine hearts, there was no enzyme deactivation for a period of two days. At the average residence time of 4 hours, the conversion of about 80 % was achieved. Inactivation of the enzyme was observed using permeabilized cells. This inactivation is described as a reversible process. Conversion of about 50 % was achieved with the remaining enzyme activity. A mathematical model that describes the production of L-malic acid, which contains the enzyme inactivation rate, was developed. Based on simulations, the used biocatalysts were compared. The results show that in the continuous production of L-malic acid, one milligram of purified enzyme corresponds to 68 g (wet weight) cells of Saccharomyces bayanus or 120 g (wet weight) cells of baker’s yeast | |
| 653 | 0 | |a L-jabučna kiselina |a Fumaraza |a Pekarski kvasac |a Enzimska aktivnost | |
| 700 | 1 | |a Zelić, Bruno | |
| 700 | 1 | |a Vasić-Rački, Đurđa | |
| 773 | 0 | |t Chemical and biochemical engineering quarterly |x 0352-9568 |g 23 (2009), 4 ; str. 519-525 |w nsk.(HR-ZaNSK)000004762 | |
| 981 | |b B03/09 | ||
| 998 | |a Luko100513 |c vol2o121009 |a mild1804 | ||
| 856 | 4 | 2 | |u http://hrcak.srce.hr/cabeq |y Chemical and Biochemical Engineering Quarterly |