Application of spectrophotometric fingerprint in cluster analysis for starch origin determination
The botanical origin of starch is of importance in industrial applications and food pro-cessing because it may influence the properties of the final product. Current microscopic methods are time-consuming. Starch consists of an origin-dependent amylose/amylopec-tin ratio. Triiodide ions bind charact...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001116164/Details |
|---|---|
| Matična publikacija: |
Food technology and biotechnology (Online) 58 (2020), 1 ; str. 5-11 |
| Glavni autori: | Sakač, Nikola (Author), Karnaš, Maja, Dobša, Jasminka, Jozanović, Marija, Gvozdić, Vlatka, Kovač, Elvira, Kraševac, Marija, Šarkanj, Bojan |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.17113/ftb.58.01.20.6239 Food technology and biotechnology (Online) Hrčak |
| LEADER | 04966naa a22004574i 4500 | ||
|---|---|---|---|
| 001 | NSK01001116164 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20211130104644.0 | ||
| 006 | m d | ||
| 007 | cr|||||||||||| | ||
| 008 | 211022s2020 ci d |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.17113/ftb.58.01.20.6239 | |
| 035 | |a (HR-ZaNSK)001116164 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 54 |2 2011 | |
| 080 | 1 | |a 66 |2 2011 | |
| 100 | 1 | |a Sakač, Nikola |4 aut | |
| 245 | 1 | 0 | |a Application of spectrophotometric fingerprint in cluster analysis for starch origin determination |h [Elektronička građa] / |c Nikola Sakač, Maja Karnaš, Jasminka Dobša, Marija Jozanović, Vlatka Gvozdić, Elvira Kovač-Andrić, Marija Kraševac Sakač, Bojan Šarkanj. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 31 jed. | ||
| 504 | |a Summary ; Sažetak. | ||
| 520 | |a The botanical origin of starch is of importance in industrial applications and food pro-cessing because it may influence the properties of the final product. Current microscopic methods are time-consuming. Starch consists of an origin-dependent amylose/amylopec-tin ratio. Triiodide ions bind characteristically to the amylose and amylopectin depending on the botanical origin of the starch. The absorbance of the starch-triiodide complex was measured for wheat, potato, corn, rye, barley, rice, tapioca and unknown origin starch, and within the different cultivars. Each starch sample had specific parameters: starch-triio-dide complex peak wavelength maximum (λmax/nm), maximum absorbance change at λmax (ΔA) and λmax shift towards the unknown origin starch sample values. The visible ab-sorption spectra (500-800 nm) for each starch sample were used as a unique fingerprint, and then elaborated by cluster analysis. The cluster analysis managed to distinguish data of two clusters, a cereal type cluster and a potato/tapioca/rice starch cluster. The cereal subclusters extensively distinguished wheat/barley/rye starches from corn starches. Data for cultivars were mostly in good agreement within the same subclaster. The proposed method that combines cluster analysis and visible absorbance data for starch-triiodide complex was able to distinguish starch of different botanical origins and cultivars within the same species. This method is simpler and more convenient than standard time-con-suming methods. | ||
| 520 | |a Botaničko podrijetlo škroba je od velike važnosti za primjenu u industriji i proizvodnji hrane jer može bitno utjecati na svojstva krajnjeg proizvoda. Trenutno dostupne mikroskopske metode za određivanje podrijetla škroba su dugotrajne. Škrob se sastoji od amiloze i amilopektina, čiji omjer ovisi o njegovom botaničkom podrijetlu. Trijodidni ioni se obično vežu za amilozu i amilopektin, a njihovo vezivanje ovisi o podrijetlu škroba. U ovom je istraživanju mjeren apsorpcijski spektar kompleksa škrob-trijodid različitih vrsta, kao što su pšenica, krumpir, kukuruz, raž, ječam, riža, tapioka i škrob nepoznatog podrijetla, te različitih sorata iste vrste. Svaki je uzorak škroba imao specifične parametre: maksimalnu valnu duljinu pika za kompleks škrob-trijodid (λmax/nm), maksimalnu promjenu apsorbancije pri λmax (ΔA) i pomak λmax prema uzorku škroba nepoznatog botaničkog podrijetla. Vidljivi je dio apsorpcijskog spektra (500-800 nm) za svaki uzorak škroba dao jedinstveni otisak, koji je zatim obrađen klaster analizom. Tom su analizom podaci razdvojeni u dva klastera, jedan klaster žitarica i drugi klaster krumpira, tapioke i riže. Unutar klastera škrobova žitarica u podklastere su se izrazito razdvojili škrobovi pšenice, ječma i raži od kukuruznih škrobova. Podaci o sortama dobro su se grupirali unutar istog podklastera. Predložena metoda, koja kombinira klaster analizu i podatke dobivene mjerenjem apsorbancije vidljivog dijela spektra kompleksa škrob-trijodid, uspješno je razvrstala škrobove na osnovi njihove pripadnosti vrstama te sortama unutar iste vrste. Metoda je jednostavnija i praktičnija za uporabu od standardnih dugotrajnih metoda. | ||
| 653 | 0 | |a Škrob |a Botaničko podrijetlo |a Klaster analiza |a Spektrofotometrijski otisak | |
| 700 | 1 | |a Karnaš, Maja |4 aut | |
| 700 | 1 | |a Dobša, Jasminka |4 aut | |
| 700 | 1 | |a Jozanović, Marija |4 aut | |
| 700 | 1 | |a Gvozdić, Vlatka |4 aut | |
| 700 | 1 | |a Kovač, Elvira |4 aut | |
| 700 | 1 | |a Kraševac, Marija |4 aut | |
| 700 | 1 | |a Šarkanj, Bojan |4 aut | |
| 773 | 0 | |t Food technology and biotechnology (Online) |x 1334-2606 |g 58 (2020), 1 ; str. 5-11 |w nsk.(HR-ZaNSK)000484955 | |
| 981 | |b Be2020 |b B04/20 | ||
| 998 | |b dalo2111 | ||
| 856 | 4 | 0 | |u https://doi.org/10.17113/ftb.58.01.20.6239 |
| 856 | 4 | 0 | |u https://www.ftb.com.hr/images/pdfarticles/2020/January-March/FTB-58-1-5.pdf |y Food technology and biotechnology (Online) |
| 856 | 4 | 0 | |u https://hrcak.srce.hr/237527 |y Hrčak |
| 856 | 4 | 1 | |y Digitalna.nsk.hr |