Spectrophotometric method for determination of Tryptophan in protein hydrolysates
A novel spectrophotometric method for determination of tryptophan content in protein hydrolysates has been developed. The reagent used is diphenylamine sulphonate which is oxidised to diphenylbenzidine sulphonic acid after reacting with sodium nitrite in the sulphuric acid medium. The unstable oxida...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01000691535/Details |
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| Matična publikacija: |
Food technology and biotechnology 45 (2007), 4 ; str. 360-366 |
| Glavni autor: | Ren, Jiaoyan (-) |
| Ostali autori: | Zhao, Mouming (-), Wang, Jinshui, Cui, Chun, Yang, Bao, biotehnolog [?] |
| Vrsta građe: | Članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
Digitalni arhiv mrežnih publikacija |
| LEADER | 02166caa a2200325 ir4500 | ||
|---|---|---|---|
| 001 | NSK01000691535 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20120103104331.0 | ||
| 007 | ta | ||
| 008 | 090224s2007 ci ||| ||eng | ||
| 035 | |9 (HR-ZaNSK)694003 | ||
| 035 | |a (HR-ZaNSK)000691535 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 042 | |a croatica | ||
| 080 | |a 57 |2 MRF 1998. | ||
| 080 | |a 54 |2 MRF 1998. | ||
| 100 | 1 | |a Ren, Jiaoyan | |
| 245 | 1 | 0 | |a Spectrophotometric method for determination of Tryptophan in protein hydrolysates / |c Jiaoyan Ren, Mouming Zhao, Jinshui Wang, Chun Cui and Bao Yang. |
| 300 | |b Ilustr. | ||
| 504 | |a Bibliografija: 27 jed | ||
| 520 | |a A novel spectrophotometric method for determination of tryptophan content in protein hydrolysates has been developed. The reagent used is diphenylamine sulphonate which is oxidised to diphenylbenzidine sulphonic acid after reacting with sodium nitrite in the sulphuric acid medium. The unstable oxidation product reacts quickly with sodium nitrite to produce a diazotized intermediate. When the diazotized intermediate is coupled with tryptophan, a pink colour product is developed, which is stable for at least 1 h at the ambient temperature. This coloured product has the absorption maximum at 522 nm and the molar absorptivity is 0.89·104 L/(mol·cm). Beer's law is obeyed in the range of 0.30–12 mg/mL. The method is applied for the analysis of tryptophan content in grass carp protein hydrolysates. Moreover, it is compared with the reversed-phase high-performance liquid chromatography analysis. There is no significant (p<0.05) difference between the two results. The method is simple, rapid and accurate compared to the previous methods | ||
| 653 | |a Aminokiseline |a Masena spektrometrija | ||
| 700 | 1 | |a Zhao, Mouming | |
| 700 | 1 | |a Wang, Jinshui | |
| 700 | 1 | |a Cui, Chun | |
| 700 | 1 | |a Yang, Bao, |c biotehnolog [?] | |
| 773 | 0 | |t Food technology and biotechnology |x 1330-9862 |g 45 (2007), 4 ; str. 360-366 |w nsk.(HR-ZaNSK)000174173 | |
| 981 | |b B12/07 | ||
| 998 | |a rado090224 |c rpeo120103 | ||
| 856 | 4 | 2 | |u http://damp.nsk.hr/index.php?show=archive&idpublication=401 |y Digitalni arhiv mrežnih publikacija |