Evaluation of potential probiotic properties of Enterococcus mundtii, its survival in boza and in situ bacteriocin production

Summary: Boza is a low-pH and low-alcohol cereal-based beverage produced in the Balkan Peninsula. Barley was cooked and prepared according to a traditional recipe and inoculated with Enterococcus mundtii ST4V (a potential probiotic and bacteriocin-producing strain), commercially produced boza, Sacch...

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Permalink: http://skupni.nsk.hr/Record/nsk.NSK01000736222/Details
Matična publikacija: Food technology and biotechnology
47 (2009), 2 ; str. 178-191
Glavni autor: Todorov, Svetoslav Dimitrov (-)
Ostali autori: Mollendorff, Johan W. von (-), Moelich, Erica, Muller, Nina, Witthuhn, R. Corli, Dicks, Leon Milner Theodore Dicks
Vrsta građe: Članak
Jezik: eng
Predmet:
Online pristup: Food Technology and Biotechnology
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035 |9 (HR-ZaNSK)738846 
035 |a (HR-ZaNSK)000736222 
040 |a HR-ZaNSK  |b hrv  |c HR-ZaNSK  |e ppiak 
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100 1 |a Todorov, Svetoslav Dimitrov 
245 1 0 |a Evaluation of potential probiotic properties of Enterococcus mundtii, its survival in boza and in situ bacteriocin production /  |c Svetoslav D. Todorov, Johan W. von Mollendorff, Erica Moelich, Nina Muller, R. Corli Witthuhn, Leon M. T. Dicks. 
300 |b Ilustr. 
504 |a Bibliografija: 51 jed 
520 8 |a Summary: Boza is a low-pH and low-alcohol cereal-based beverage produced in the Balkan Peninsula. Barley was cooked and prepared according to a traditional recipe and inoculated with Enterococcus mundtii ST4V (a potential probiotic and bacteriocin-producing strain), commercially produced boza, Saccharomyces cerevisiae, and a combination of strain E. mundtii ST4V and Saccharomyces cerevisiae. Fermentation was carried out at 37 °C for 3 h. The organoleptic properties of fermented products were evaluated by a qualified taste panel. No significant differences in rheological properties were observed, suggesting that E. mundtii ST4V had no effect on the quality of the final product. Microbial cell numbers remained relatively unchanged during one week of storage. The preservative properties of bacteriocin ST4V were evaluated by contaminating boza with Lactobacillus sakei DSM 20017. Changes in microbial populations were monitored by using classical microbiological methods, PCR with species-specific primers and denaturing gradient gel electrophoresis (DGGE). Adsorption of bacteriocin ST4V to target cells is pH-dependent, with the highest adsorption (88 %) recorded at pH=8.0 and pH=10.0. Maximum adsorption of bacteriocin ST4V (75 %) to Enterococcus faecalis and Listeria innocua was recorded at 25 to 37 °C. Growth of E. mundtii ST4V was inhibited only by a few antibiotics and anti-inflammatory medicaments, suggesting that the strain may be used as a probiotic by individuals receiving medical treatment 
653 0 |a Enterococcus mundtii  |a Boza  |a Bakteriocin  |a Probiotik 
700 1 |a Mollendorff, Johan W. von 
700 1 |a Moelich, Erica 
700 1 |a Muller, Nina 
700 1 |a Witthuhn, R. Corli 
700 1 |a Dicks, Leon Milner Theodore Dicks 
773 0 |t Food technology and biotechnology  |x 1330-9862  |g 47 (2009), 2 ; str. 178-191  |w nsk.(HR-ZaNSK)000174173 
981 |b B03/09 
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856 4 2 |u http://hrcak.srce.hr/ftb  |y Food Technology and Biotechnology