Lipid composition of Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source
Research background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-L-methi...
| Permalink: | http://skupni.nsk.hr/Record/nsk.NSK01001116144/Details |
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| Matična publikacija: |
Food technology and biotechnology (Online) 58 (2020), 2 ; str. 203-213 |
| Glavni autori: | Križanović, Stela (Author), Stanzer, Damir, Mrvčić, Jasna, Hanousek-Čiča, Karla, Kralj, Elizabeta, Čanadi Jurešić, Gordana |
| Vrsta građe: | e-članak |
| Jezik: | eng |
| Predmet: | |
| Online pristup: |
https://doi.org/10.17113/ftb.58.02.20.6540 Food technology and biotechnology (Online) Hrčak |
| LEADER | 05136naa a22004214i 4500 | ||
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| 001 | NSK01001116144 | ||
| 003 | HR-ZaNSK | ||
| 005 | 20211129110351.0 | ||
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| 007 | cr|||||||||||| | ||
| 008 | 211022s2020 ci d |o |0|| ||eng | ||
| 024 | 7 | |2 doi |a 10.17113/ftb.58.02.20.6540 | |
| 035 | |a (HR-ZaNSK)001116144 | ||
| 040 | |a HR-ZaNSK |b hrv |c HR-ZaNSK |e ppiak | ||
| 041 | 0 | |a eng |b hrv |b eng | |
| 042 | |a croatica | ||
| 044 | |a ci |c hr | ||
| 080 | 1 | |a 663 |2 2011 | |
| 100 | 1 | |a Križanović, Stela |4 aut | |
| 245 | 1 | 0 | |a Lipid composition of Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source |h [Elektronička građa] / |c Stela Križanović, Damir Stanzer, Jasna Mrvčić, Karla Hanousek-Čiča, Elizabeta Kralj, Gordana Čanadi Jurešić. |
| 300 | |b Graf. prikazi. | ||
| 504 | |a Bibliografija: 45 jed. | ||
| 504 | |a Summary ; Sažetak. | ||
| 520 | |a Research background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-L-methionine (SAM) production. Experimental approach. The batch cultivation of M12 yeast was carried out under aerobic conditions in a laboratory bioreactor with glycerol as carbon source, and with pulsed addition of methionine. Glycerol and SAM content were monitored by high-performance liquid chromatography, while fatty acid composition of different lipid classes, separated by solid phase extraction, was determined by gas chromatography.Results and conclusion. Despite the reduced amount of ergosterol in yeast cells, thanks to the reorganized lipid metabolism, M12 strain achieved high biomass yield and SAM production. Neutral lipids prevailed (making more than 75 % of total lipids), but their content and composition differed significantly in the two tested types of yeast. Unsaturated and C18 fatty acids prevailed in both the M12 strain and wild type. In all fractions except free fatty acids, the index of unsaturation in M12 strain was lower than in the wild strain. Our tested strain adjusts itself by changing the content of lipids (mainly phospholipids, sterols and sterol esters), and with desaturation adjustments, to maintain proper functioning and fulfil increased energy needs.Novelty and scientific contribution. Reorganization of S. stipitis lipid composition caused by blocking the metabolic pathway of ergosterol synthesis was presented. A simple scheme of actual lipid metabolism during active SAM production in S. stipitis, grown on glycerol was constructed and shown. This fundamental knowledge of lipid metabolic pathways will be a helpful tool in improving S. stipitis as an expression host and a model organism, opening new perspectives for its applied research. | ||
| 520 | |a Pozadina istraživanja. U ovom je istraživanju određen sadržaj i sastav lipida u soju kvasca Sheffersomyces stipitis M12 s narušenom biosintezom ergosterola, uzgojenom na glicerolu kao izvoru ugljika. Zaustavljanje biosintetskog puta ergosterola jedna je od metoda koja se u novije vrijeme primjenjuje za povećanje udjela S-adenozil-L-metionina (SAM) u biomasi kvasca. Eksperimentalni pristup. Šaržni je uzgoj kvasca M12 proveden aerobno u laboratorijskom bioreaktoru, s glicerolom kao izvorom ugljika i uz pulsni dodatak metionina. Koncentracija glicerola i maseni udjel SAM-a određeni su tekućinskom kromatografijom visoke djelotvornosti, dok je masnokiselinski sastav svih klasa lipida, odijeljenih ekstrakcijom na čvrstoj fazi, utvrđen plinskom kromatografijom. Rezultati i zaključci. Usprkos manjem udjelu ergosterola u kvascu M12, zbog reorganizacije metabolizma lipida ostvareni su veliki prirast biomase i prinos SAM-a. U tom soju kvasca prevladavali su neutralni lipidi (više od 75 % ukupnih lipida), no njihov sadržaj i sastav bio je značajno izmijenjen u odnosu na divlji tip kvasca. Nezasićene i C18 masne kiseline prevladavale su u obje vrste kvasca. U svim je frakcijama, osim u frakciji slobodnih masnih kiselina, indeks nezasićenosti bio niži u soju M12 u usporedbi s divljim tipom kvasca. Soj M12 je u promijenjenim uvjetima rasta nesmetano funkcionirao i zadovoljio sve svoje energetske potrebe mijenjanjem sastava lipida (osobito fosfolipida, sterola i sterolnih estera) i ugađanjem njihove nezasićenosti. | ||
| 653 | 0 | |a Kvasac |a Lipidi |a Sheffersomyces stipitis |a Glicerol | |
| 700 | 1 | |a Stanzer, Damir |4 aut | |
| 700 | 1 | |a Mrvčić, Jasna |4 aut | |
| 700 | 1 | |a Hanousek-Čiča, Karla |4 aut | |
| 700 | 1 | |a Kralj, Elizabeta |4 aut | |
| 700 | 1 | |a Čanadi Jurešić, Gordana |4 aut | |
| 773 | 0 | |t Food technology and biotechnology (Online) |x 1334-2606 |g 58 (2020), 2 ; str. 203-213 |w nsk.(HR-ZaNSK)000484955 | |
| 981 | |b Be2020 |b B04/20 | ||
| 998 | |b dalo2111 | ||
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| 856 | 4 | 1 | |y Digitalna.nsk.hr |